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KMID : 0379119930210010009
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Korean Journal of Mycology
1993 Volume.21 No. 1 p.9 ~ p.15
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Fusion Hybrid and Carotenoid Formation from the Yeast , Phaffia rhodozyma
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Abstract
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The cell fusants were constructed from complementary auxotrophic mutants of Phffia rhodozyma. The nuclear fusion of the fusants was demonstrated by several techniques including comparison of cell volume, estimation of DNA content and nuclear staining. The cell fusants were very stable for succeeding transfer culture on complex medium for more than one year. Malt extract (1%, w/v) and abscisic acid(1 mM) increased the carotenoid formation whereas gibberellic acid(5 mM) and riboflavin(0.1 mM) decreased the corresponding content.
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